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Title: Pc16a, the first characterized peptide from Conus pictus venom, shows a novel disulfide connectivity
Authors: Van Der Haegen, Annelies ×
Peigneur, Steve
Dyubankova, Natalia
Möller, Carolina
Mari, Frank
Diego-Garcia E, Elia
Naudé, Ryno
Lescrinier, Eveline
Herdewijn, Piet
Tytgat, Jan #
Issue Date: Mar-2012
Publisher: Elsevier
Series Title: Peptides vol:34 issue:1 pages:106-113
Abstract: A novel conotoxin, pc16a, was isolated from the venom of Conus pictus. This is the first peptide characterized from this South-African cone snail and it has only 11 amino acid residues, SCSCKRNFLCC*, with the rare cysteine framework XVI and a monoisotopic mass of 1257.6Da. Two peptides were synthesized with two possible conformations: globular (pc16a_1) and ribbon (pc16a_2). pc16a_1 co-eluted with the native peptide, which indicates a disulfide connectivity I-III, II-IV. The structure of pc16a_1 was determined by NMR. Both synthetic peptides were used to elucidate the biological activity. Bioassays were performed on crickets, ghost shrimps, larvae of the mealworm beetle and mice, but no effect was seen. Using two-electrode voltage clamp, a range of voltage-gated ion channels (Na(v) and K(v)) and nicotinic acetylcholine receptors were screened, but again no activity was found. Hence, the specific target of pc16a still remains to be discovered.
URI: 
ISSN: 0196-9781
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Medicinal Chemistry
Toxicology and Pharmacology
× corresponding author
# (joint) last author

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