Infection, Genetics and Evolution vol:13 pages:27-35
During the 2008-2009 rotavirus season, 10 G3P rotavirus strains were isolated for the first time in Belgium, while an outbreak of G2P strains occurred in the USA in 2005-2006. Partial sequencing of the 11 genome segments of the 10 Belgian G3P strains revealed a clonal origin. Two of these strains, and a G2P strain representative of the American outbreak, were selected and sequenced completely to analyze their evolutionary relationships. Genetic analysis revealed that all strains possessed a DS-1-like genotype constellation. The 2 Belgian G3P strains showed >99% sequence identity at the nucleotide level and the American G2P strain was phylogenetically closely related to the Belgian P strains. These data suggest that reassortment(s) involving VP7 occurred recently, and that the prevalence of DS-1-like P rotavirus strains need to be closely monitored because the currently licensed RVA vaccines contain neither the P genotype nor strains with a complete human DS-1 genotype constellation.