ITEM METADATA RECORD
Title: Hepatic alpha-oxidation of phytanic acid - A revised pathway
Authors: Van Veldhoven, Paul P ×
Mannaerts, Guy
Casteels, Minne
Croes, K #
Issue Date: Jan-1999
Publisher: Kluwer academic / plenum publ
Series Title: Current views of fatty acid oxidation and ketogenesis vol:466 pages:273-281
Abstract: Synthetic 3-methyl-branched chain fatty acids were used to decipher the breakdown of phytanic acid. Based on results obtained in intact or permeabilized rat hepatocytes, rat liver homogenates or subcellular fractions, a revised a-oxidation pathway is proposed which appears to be functioning in man as well. In a first step, the 3-methyl-branched chain fatty acid is activated by an acyl-CoA synthetase. This reaction requires CoA, ATP and Mg2+. Subsequently, the acyl-CoA ester is hydroxylated at position 2 by a peroxisomal dioxygenase. This step is dependent on alpha-oxoglutarate. ascorbate (or glutathione), Fe2+ and O-2. The 2-hydroxy-3-methylacyl-CoA intermediate is cleaved by a peroxisomal lyase to formyl-CoA and a 2-methyl-branched fatty aldehyde. Formyl-CoA is (partly enzymically) hydrolyzed to formate, which is then converted, most likely in the cytosol, to CO2. In the presence of NAD(+), the aldehyde is dehydrogenated to a 2-methyl-branched fatty acid, presumably by a peroxisomal aldehyde dehydrogenase. This acid can-after activation-be degraded via a D-specific peroxisomal beta-oxidation system.
ISSN: 0065-2598
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Pharmacology Section (-)
Laboratory of Lipid Biochemistry and Protein Interactions
Department of Pharmaceutical & Pharmacological Sciences - miscellaneous
× corresponding author
# (joint) last author

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