Title: Macrophage skewing by Phd2 haplodeficiency prevents ischaemia by inducing arteriogenesis
Authors: Takeda, Yukiji
Costa, Sandra
Delamarre, Estelle
Roncal, Carmen
Leite de Oliveira, Rodrigo
Squadrito, Mario Leonardo
Finisguerra, Veronica
Deschoemaeker, Sofie
Bruyère, Françoise
Wenes, Mathias
Hamm, Alexander
Serneels, Jens
Magat, Julie
Bhattacharyya, Tapan
Anisimov, Andrey
Jordan, Benedicte F
Alitalo, Kari
Maxwell, Patrick
Gallez, Bernard
Zhuang, Zhen W
Saito, Yoshihiko
Simons, Michael
De Palma, Michele
Mazzone, Max # ×
Issue Date: Nov-2011
Publisher: Nature Publishing Group
Series Title: Nature vol:479 issue:7371 pages:122-U153
Article number: 10.1038/nature10507
Abstract: PHD2 serves as an oxygen sensor that rescues blood supply by regulating vessel formation and shape in case of oxygen shortage. However, it is unknown whether PHD2 can influence arteriogenesis. Here we studied the role of PHD2 in collateral artery growth by using hindlimb ischaemia as a model, a process that compensates for the lack of blood flow in case of major arterial occlusion. We show that Phd2 (also known as Egln1) haplodeficient (Phd2(+/-)) mice displayed preformed collateral arteries that preserved limb perfusion and prevented tissue necrosis in ischaemia. Improved arteriogenesis in Phd2(+/-) mice was due to an expansion of tissue-resident, M2-like macrophages and their increased release of arteriogenic factors, leading to enhanced smooth muscle cell (SMC) recruitment and growth. Both chronic and acute deletion of one Phd2 allele in macrophages was sufficient to skew their polarization towards a pro-arteriogenic phenotype. Mechanistically, collateral vessel preconditioning relied on the activation of canonical NF-κB pathway in Phd2(+/-) macrophages. These results unravel how PHD2 regulates arteriogenesis and artery homeostasis by controlling a specific differentiation state in macrophages and suggest new treatment options for ischaemic disorders.
ISSN: 0028-0836
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Tumor Inflammation and Angiogenesis (Vesalius Research Center) (+)
Laboratory of Angiogenesis and Vascular Metabolism (Vesalius Research Center) (+)
× corresponding author
# (joint) last author

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