Title: Identification of EPS-degrading activity within the tail spikes of the novel Pseudomonas putida phage AF
Authors: Cornelissen, Anneleen
Ceyssens, Pieter-Jan
Krylov, Victor N
Noben, Jean-Paul
Volckaert, Guido
Lavigne, Rob # ×
Issue Date: Dec-2012
Publisher: Academic Press
Series Title: Virology vol:434 issue:2 pages:251-256
Article number: S0042-6822(12)00471-0
Abstract: We report the study of phage AF, the first member of the canonical lambdoid phage group infecting Pseudomonas putida. Its 42.6kb genome is related to the "epsilon15-like viruses" and the "BPP-1-like viruses", a clade of bacteriophages shaped by extensive horizontal gene transfer. The AF virions display exopolysaccharide (EPS)-degrading activity, which originates from the action of the C-terminal domain of the tail spike (Gp19). This protein shows high similarity to the tail spike of the T7-like P. putida-infecting phage φ15. These unrelated phages have an identical host spectrum and EPS degradation characteristics, designating the C-terminal part of Gp19 as sole determinant for these functions. While intact AF particles have biofilm-degrading properties, Gp19 and non-infectious AF particles do not, emphasizing the role of phage amplification in biofilm degradation.
ISSN: 0042-6822
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Gene Technology (-)
× corresponding author
# (joint) last author

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