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Title: First proteomic analysis of Legionella pneumophila based on its developing genome sequence
Authors: Lebeau, Ilya ×
Lammertyn, Elke
De Buck, Emmy
Maes, Liesbeth
Geukens, Nick
Van Mellaert, Lieve
Arckens, Lut
Anné, Jozef
Clerens, Stefan #
Issue Date: Jan-2005
Publisher: Elsevier science bv
Series Title: Research in microbiology vol:156 issue:1 pages:119-29
Abstract: The first proteomic analysis of the respiratory pathogen Legionella pneumophila ATCC 33152 is presented in this report. Two-dimensional gel electrophoresis of total cell extracts was carried out. In total, 130 protein spots were identified by matrix-assisted laser desorption/ionisation time-of-flight mass spectrometry (MS) or by quadruple time-of-flight tandem MS, including proteins correlated with virulence. For the first time, proteins of L. pneumophila were identified using mass spectrometric methods and mapped on a two-dimensional gel; this will be of considerable use for comparison of protein expression profiles of L. pneumophila wild-type and knock-out mutant strains and of L. pneumophila grown under different conditions.
URI: 
ISSN: 0923-2508
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Bacteriology (Rega Institute)
Animal Physiology and Neurobiology Section - miscellaneous
Research Group Neuroplasticity and Neuroproteomics (-)
Laboratory of Virology and Chemotherapy (Rega Institute)
Academic Center for General Practice
× corresponding author
# (joint) last author

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