Title: Amorphous silica nanoparticles promote monocyte adhesion to human endothelial cells: Size-dependent effect
Authors: Napierska, Dorota
Quarck, Rozenn
Thomassen, Leen
Lison, Dominique
Martens, Johan
Delcroix, Marion
Nemery, Benoit
Hoet, Peter # ×
Issue Date: Feb-2013
Publisher: Wiley
Series Title: Small vol:9 issue:3 pages:430-438
Abstract: There is evidence that nanoparticles can induce endothelial dysfunction. Here, the effect of monodisperse amorphous silica nanoparticles (SiO(2) -NPs) of different diameters on endothelial cells function is examined. Human endothelial cell line (EA.hy926) or primary human pulmonary artery endothelial cells (hPAEC) are seeded in inserts introduced or not above triple cell co-cultures (pneumocytes, macrophages, and mast cells). Endothelial cells are incubated with SiO(2) -NPs at non-cytotoxic concentrations for 12 h. A significant increase (up to 2-fold) in human monocytes adhesion to endothelial cells is observed for 18 and 54 nm particles. Exposure to SiO(2) -NPs induces protein expression of adhesion molecules (ICAM-1 and VCAM-1) as well as significant up-regulation in mRNA expression of ICAM-1 in both endothelial cell types. Experiments performed with fluorescent-labelled monodisperse amorphous SiO(2) -NPs of similar size evidence nanoparticle uptake into the cytoplasm of endothelial cells. It is concluded that exposure of human endothelial cells to amorphous silica nanoparticles enhances their adhesive properties. This process is modified by the size of the nanoparticle and the presence of other co-cultured cells.
ISSN: 1613-6810
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Occupational, Environmental and Insurance Medicine (-)
Microbial and Molecular Systems - miscellaneous
Centre for Surface Chemistry and Catalysis
Formerly "Departement Industriƫle Wetenschappen - TM M"
Environment and Health - miscellaneous
Sustainable Chemical Process Technology TC, Technology Campus Diepenbeek
Sustainable Chemical Process Technology TC
× corresponding author
# (joint) last author

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