Synaptic Vesicles Studied by SAXS: Derivation and Validation of a Model Form Factor
Castorph, Simon Arleth, Lise Sztucki, Michael Vainio, U. Ghosh, Sajal Kumar Holt, Matthew × Salditt, Tim #
Institute of Physics Publishing Ltd.
Journal of Physics - Conference Series vol:247 pages:1-17
XIV International Conference on Small-Angle Scattering (SAS09) edition:247 location:Oxford, UK date:13-18 September 2009
We discuss different spherically symmetric and anisotropic form factor models and
test them against high resolution synchrotron based small-angle x-ray scattering (SAXS) data
from synaptic vesicles (SVs), isolated from rat brain. Anisotropy of the model form factors is
found to be a key ingredient for the description of the native synaptic vesicle structure. We
describe changes in structural parameters due to protease digestion of SVs, and present SAXS
data of SVs recorded under different pH conditions.