Molecular Anatomy of a Trafficking Organelle

Takamori, Shigeo; Holt, Matthew; Stenius, Katinka; Lemke, Edward A; Grønborg, Mads; Riedel, Dietmar; Urlaub, Henning; Schenck, Stephan; Brügger, Britta; Ringler, Philippe; Müller, Shirley A; Rammner, Burkhard; Gräter, Frauke; Hub, Jochen S; De Groot, Bert L; Mieskes, Gottfried; Moriyama, Yoshinori; Klingauf, Jürgen; Grubmüller, Helmut; Heuser, John; Wieland, Felix; Jahn, Reinhard

doi:10.1016/j.cell.2006.10.030

Molecular Anatomy of a Trafficking Organelle

Author:

Takamori, Shigeo

Holt, Matthew ; Stenius, Katinka ; Lemke, Edward A ; Grønborg, Mads ; Riedel, Dietmar ; Urlaub, Henning ; Schenck, Stephan ; Brügger, Britta ; Ringler, Philippe ; Müller, Shirley A ; Rammner, Burkhard ; Gräter, Frauke ; Hub, Jochen S ; De Groot, Bert L ; Mieskes, Gottfried ; Moriyama, Yoshinori ; Klingauf, Jürgen ; Grubmüller, Helmut ; Heuser, John ; Wieland, Felix ; Jahn, Reinhard

Keywords:

Synaptic Vesicles, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Cell Biology, VESICULAR GLUTAMATE TRANSPORTER, CLATHRIN-COATED VESICLES, SYNAPTIC VESICLES, SYNAPSIN-I, PROTEIN-I, MEMBRANE, SNARE, IDENTIFICATION, DEFINES, BRAIN, Animals, Lipids, Models, Molecular, Protein Transport, Proteins, Rats, Spectrometry, Fluorescence, 06 Biological Sciences, 11 Medical and Health Sciences, Developmental Biology, 31 Biological sciences, 32 Biomedical and clinical sciences

Abstract:

Membrane traffic in eukaryotic cells involves transport of vesicles that bud from a donor compartment and fuse with an acceptor compartment. Common principles of budding and fusion have emerged, and many of the proteins involved in these events are now known. However, a detailed picture of an entire traffickingorganelle is not yet available. Using synaptic vesicles as a model, we have now determined the protein and lipid composition; measured vesicle size, density, and mass; calculated the average protein and lipid mass per vesicle; and determined the copy number of more than a dozen major constituents. A model has been constructed that integrates all quantitative data and includes structural models of abundant proteins. Synaptic vesicles are dominated by proteins, possess a surprising diversity of trafficking proteins, and, with the exception of the V-ATPase that is present in only one to two copies, contain numerous copies of proteins essential for membrane traffic and neurotransmitter uptake.