ITEM METADATA RECORD
Title: The EMT regulator Zeb2/Sip1 is essential for murine embryonic hematopoietic stem/progenitor cell differentiation and mobilization
Authors: Goossens, Steven
Janzen, Viktor
Bartunkova, Sonia
Yokomizo, Tomomasa
Drogat, Benjamin
Crisan, Mihaela
Haigh, Katharina
Seuntjens, Eve
Umans, Lieve
Riedt, Tamara
Bogaert, Pieter
Haenebalcke, Lieven
Berx, Geert
Dzierzak, Elaine
Huylebroeck, Danny
Haigh, Jody J # ×
Issue Date: May-2011
Publisher: W.B. Saunders
Series Title: Blood vol:117 issue:21 pages:5620-5630
Abstract: Zeb2 (Sip1/Zfhx1b) is a member of the zinc-finger E-box-binding (ZEB) family of transcriptional repressors previously demonstrated to regulate epithelial-to-mesenchymal transition (EMT) processes during embryogenesis and tumor progression. We found high Zeb2 mRNA expression levels in HSCs and hematopoietic progenitor cells (HPCs), and examined Zeb2 function in hematopoiesis through a conditional deletion approach using the Tie2-Cre and Vav-iCre recombination mouse lines. Detailed cellular analysis demonstrated that Zeb2 is dispensable for hematopoietic cluster and HSC formation in the aorta-gonadomesonephros region of the embryo, but is essential for normal HSC/HPC differentiation. In addition, Zeb2-deficient HSCs/HPCs fail to properly colonize the fetal liver and/or bone marrow and show enhanced adhesive properties associated with increased β1 integrin and Cxcr4 expression. Moreover, deletion of Zeb2 resulted in embryonic (Tie2-Cre) and perinatal (Vav-icre) lethality due to severe cephalic hemorrhaging and decreased levels of angiopoietin-1 and, subsequently, improper pericyte coverage of the cephalic vasculature. These results reveal essential roles for Zeb2 in embryonic hematopoiesis and are suggestive of a role for Zeb2 in hematopoietic-related pathologies in the adult.
URI: 
ISSN: 0006-4971
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Embryo and Stemcells (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
The EMT regulator Zeb2:Sip1 is essential for murine embryonic hematopoietic stem:progenitor cell differentiation and mobilization.pdf Published 832KbAdobe PDFView/Open

 


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science