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Title: Synaptic vesicles studied by dynamic light scattering
Authors: Castorph, S ×
Schwarz Henriques, S
Holt, Matthew
Riedel, D
Jahn, R
Salditt, T #
Issue Date: Jun-2011
Publisher: Springer
Series Title: European Physical Journal E, Soft Matter vol:34 issue:6 pages:1-11
Article number: 10.1140/epje/i2011-11063-2
Abstract: The size polydispersity distribution of synaptic vesicles (SVs) is characterized under quasi-physiological conditions by dynamic light scattering (DLS). Highly purified fractions of SVs obtained from rat brain still contain a small amount of larger contaminant structures, which can be quantified by DLS and further reduced by asymmetric-flow field-flow (AFFF) fractionation. The intensity autocorrelation functions g (2)(τ) recorded from these samples are analyzed by a constrained regularization method as well as by an alternative direct modeling approach. The results are in quantitative agreement with the polydispersity obtained from cryogenic electron microscopy of vitrified SVs. Next, different vesicle fusion assays based on samples composed of SVs and small unilamellar proteoliposomes with the fusion proteins syntaxin 1 and SNAP-25A are characterized by DLS. The size increase of the proteoliposomes due to SNARE-dependent fusion with SVs is quantified by DLS under quasi-physiological conditions.
ISSN: 1292-8941
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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