Title: Measuring Calcium-induced structural changes in lipid monolayers: implications for synaptic vesicle exocytosis
Authors: Ghosh, Sajal Kumar
Castorph, Simon
Konovalov, Oleg
Salditt, Tim
Jahn, Reinhard
Holt, Matthew # ×
Issue Date: Mar-2012
Publisher: Biophysical Society
Series Title: Biophysical Journal vol:102 issue:6 pages:1394-1402
Abstract: Synaptic vesicles (SVs) are small, membrane-bound organelles that are found in the synaptic terminal of neurons. Although tremendous progress has been made in understanding the protein machinery that drives fusion of SVs with the presynaptic membrane, little progress has been made in understanding changes in the membrane structure that accompany this process. We used lipid monolayers of defined composition to mimic biological membranes, which were probed by x-ray reflectivity and grazing incidence x-ray diffraction. These techniques allowed us to successfully monitor structural changes in the membranes at molecular level, both in response to injection of SVs in the subphase below the monolayer, as well as to physiological cues involved in neurotransmitter release, such as increases in the concentration of the membrane lipid PIP(2), or addition of physiological levels of Ca(2+). Such structural changes may well modulate vesicle fusion in vivo.
ISSN: 0006-3495
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
measuring ca2.pdfOA article Published 1000KbAdobe PDFView/Open Request a copy

These files are only available to some KU Leuven Association staff members


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science