Title: Cooperation of Syd-1 with Neurexin synchronizes pre- with postsynaptic assembly
Authors: Owald, David
Khorramshahi, Omid
Gupta, Varun K
Banovic, Daniel
Depner, Harald
Fouquet, Wernher
Wichmann, Carolin
Mertel, Sara
Eimer, Stefan
Reynolds, Eric
Holt, Matthew
Aberle, Hermann
Sigrist, Stephan J # ×
Issue Date: Aug-2012
Publisher: Nature America Inc.
Series Title: Nature Neuroscience vol:15 issue:9 pages:1219-1226
Article number: 10.1038/nn.3183
Abstract: Synapse formation and maturation requires bidirectional communication across the synaptic cleft. The trans-synaptic Neurexin-Neuroligin complex can bridge this cleft, and severe synapse assembly deficits are found in Drosophila melanogaster neuroligin (Nlg1, dnlg1) and neurexin (Nrx-1, dnrx) mutants. We show that the presynaptic active zone protein Syd-1 interacts with Nrx-1 to control synapse formation at the Drosophila neuromuscular junction. Mutants in Syd-1 (RhoGAP100F, dsyd-1), Nrx-1 and Nlg1 shared active zone cytomatrix defects, which were nonadditive. Syd-1 and Nrx-1 formed a complex in vivo, and Syd-1 was important for synaptic clustering and immobilization of Nrx-1. Consequently, postsynaptic clustering of Nlg1 was affected in Syd-1 mutants, and in vivo glutamate receptor incorporation was changed in Syd-1, Nrx-1 and Nlg1 mutants. Stabilization of nascent Syd-1-Liprin-α (DLiprin-α) clusters, important to initialize active zone formation, was Nlg1 dependent. Thus, cooperation between Syd-1 and Nrx-1-Nlg1 seems to orchestrate early assembly processes between pre- and postsynaptic membranes, promoting avidity of newly forming synaptic scaffolds.
ISSN: 1097-6256
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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