Title: Mouse macrophage derived monocyte chemotactic protein-3: cDNA cloning and identification as MARC/FIC
Authors: Thirion, S ×
Nys, G
Fiten, P
Masure, S
Van Damme, Jozef
Opdenakker, Ghislain #
Issue Date: Jun-1994
Publisher: Academic press inc jnl-comp subscriptions
Series Title: Biochemical and Biophysical Research Communications vol:201 issue:2 pages:493-9
Abstract: When the mouse macrophage cell line WEHI-3 is triggered with LPS it produces proteases and secondary cytokines including interleukin-6 and chemokines. In an attempt to isolate the mouse homologue of the human monocyte chemotactic protein-3 (MCP-3), a cDNA library from LPS-stimulated WEHI-3 cells was screened with the full-size human MCP-3 cDNA. The longest cDNA out of several positive clones was sequenced and encoded a protein of 97 residues. Except for a third codon letter mismatch it was identical to the mouse MARC cDNA and encoded the MARC protein. The murine Fic cDNA, which encodes a Marc-mutant protein with an arginine substitution for alanine, was not identified in the other sequenced homologous isolates. Similar to the human system, in which MCP-3 is most related to MCP-1, MURINE MCP-3 was found to be more homologous to mouse MCP-1/JE than to other murine C-C chemokines. We therefore postulate that MARC/FIC is the mouse MCP-3.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Immunology (Rega Institute)
Academic Center for General Practice
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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