Author:

Keywords:

Science & Technology, Life Sciences & Biomedicine, Cell Biology, Hematology, Immunology, inflammation, skin, keratinocytes, MEDIATED TISSUE DESTRUCTION, KERATINOCYTE-DERIVED IL-10, SKIN ALLOGRAFT SURVIVAL, EXPRESSING INTERLEUKIN-10, CROHNS-DISEASE, T-CELLS, MICE, SUPERNATANTS, INFLAMMATION, LANGERHANS, Animals, Graft Rejection, Graft Survival, Interleukin-10, Keratinocytes, Male, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, Knockout, Models, Animal, Skin Transplantation, Transplantation, Isogeneic, 0601 Biochemistry and Cell Biology, 1107 Immunology, 3204 Immunology

Abstract:

We performed a comparative study and evaluated cellular infiltrates and anti-inflammatory cytokine production at different time-points after syngeneic or allogeneic skin transplantation. We observed an early IL-10 production in syngeneic grafts compared with allografts. This observation prompted us to investigate the role of IL-10 in isograft acceptance. For this, we used IL-10 KO and WT mice to perform syngeneic transplantation, where IL-10 was absent in the graft or in the recipient. The majority of syngeneic grafts derived from IL-10 KO donors did not engraft or was only partially accepted, whereas IL-10 KO mice transplanted with skin from WT donors accepted the graft. We evaluated IL-10 producers in the transplanted skin and observed that epithelial cells were the major source. Taken together, our data show that production of IL-10 by donor cells, but not by the recipient, is determinant for graft acceptance and strongly suggest that production of this cytokine by keratinocytes immediately upon transplantation is necessary for isograft survival.