Title: Pulmonary inflammation in mice with collagen-induced arthritis is conditioned by complete Freund's adjuvant and regulated by endogenous IFN-γ
Authors: Schurgers, Evelien ×
Mertens, Freya
Vanoirbeek, Jeroen
Put, St├ęphanie
Mitera, Tania
De Langhe, Ellen
Billiau, Alfons
Hoet, Peter
Nemery, Benoit
Verbeken, Erik
Matthys, Patrick #
Issue Date: Dec-2012
Publisher: Wiley-VCH
Series Title: European Journal of Immunology vol:42 issue:12 pages:3223-3234
Abstract: Following immunization with collagen II (CII) in complete Freund's adjuvant (CFA), DBA/1 mice develop arthritis of major joints. This collagen-induced arthritis (CIA) is used as a model for rheumatoid arthritis (RA) in man. Inflammatory changes in lung tissue commonly occur in RA. However, evidence for pulmonary inflammation in CIA is scarce and ambiguous. Here we demonstrate pulmonary inflammation accompanying CIA in wild-type DBA/1 mice. In IFN-γ receptor-deficient (IFN-γR KO) mice, inflammation was more frequent and more severe. Injection of CFA only (without CII) proved to be as efficient in eliciting pulmonary inflammation as immunization with CFA+CII, though being less effective in causing arthritis. Significant correlation in severity between joint and pulmonary involvement could not be demonstrated. Macroscopic, microscopic and functional characteristics of pulmonary inflammation in the mice resembled those seen in human RA. Increased inflammation in IFN-γR KO mice was accompanied by augmented expression of various cytokines and chemokines, as measured by RT-PCR on affected tissue. Treatment with a TNF-α inhibitor ameliorated lung pathology. We conclude that CIA in DBA/1 mice is accompanied by pulmonary inflammation. Although both disease processes are kept in check by endogenous IFN-γ, lack of strict parallelism indicates that overlap in their pathogeneses is partial.
ISSN: 0014-2980
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Occupational, Environmental and Insurance Medicine (-)
Laboratory of Immunobiology (Rega Institute)
Translational Cell & Tissue Research
Stem Cell Biology and Embryology (+)
Environment and Health - miscellaneous
Department of Development and Regeneration - miscellaneous (+)
× corresponding author
# (joint) last author

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