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Title: Gelatinase B/matrix metalloproteinase-9 provokes cataract by cleaving lens betaB1 crystallin
Authors: Descamps, Francis ×
Martens, Erik
Proost, Paul
Starckx, Sofie
Van den Steen, Philippe
Van Damme, Jozef
Opdenakker, Ghislain #
Issue Date: Jan-2005
Publisher: The Federation of American Societies for Experimental Biology
Series Title: FASEB Journal vol:19 issue:1 pages:29-35
Abstract: Cataract is a common cause of blindness and results from destruction of the microarchitecture of the lens. It is observed in many genetic syndromes, infections, inflammatory diseases and during aging. Fluctuations in lens density and light scattering by altered refraction index form the physical basis for this process, but the pathogenesis is poorly understood. Increased levels of gelatinase B/matrix metalloproteinase-9 have been reported for cataract-associated disorders such as eye inflammation and diabetes. We demonstrate that incubation of lenses with gelatinase B leads immediately to cataract. In complete eye extracts, betaB1 crystallin was identified as the major gelatinase B substrate by combination of proteomics, mass spectrometry, and Edman degradation analysis. The cleavage of betaB1 crystallin was also observed in vivo after endogenous gelatinase B-induction by the chemokine granulocyte chemotactic protein-2 in wild-type mice but not in gelatinase B-/- mice.
ISSN: 0892-6638
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Immunology (Rega Institute)
Laboratory of Immunobiology (Rega Institute)
Biochemistry Section (Medicine) (-)
Leuven Language Institute (ILT)
Faculty of Arts, Campus Kulak Kortrijk
Faculty of Pharmaceutical Sciences - miscellaneous
× corresponding author
# (joint) last author

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