Title: The use of the cMyc epitope tag can be problematic for protein detection in Legionella pneumophila
Authors: De Buck, Emmy ×
Lebeau, Ilya
Van Mellaert, Lieve
Geukens, Nick
Anné, Jozef
Lammertyn, Elke #
Issue Date: Oct-2004
Series Title: Journal of microbiological methods vol:59 issue:1 pages:131-4
Abstract: In various recent experiments with respect to protein secretion by L. pneumophila, protein detection based on the cMyc-tag seemed to be problematic. For one specific protein, we studied the reason for this problem in more detail by comparing protein expression in the presence and absence of the cMyc-tag. We demonstrated a decrease in the amount of mRNA transcript when the cMyc coding sequence is present. We can conclude that this epitope tag is not a preferential tag for protein detection in L. pneumophila.
ISSN: 0167-7012
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Bacteriology (Rega Institute)
Laboratory of Virology and Chemotherapy (Rega Institute)
Academic Center for General Practice
× corresponding author
# (joint) last author

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