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Title: Phosphoinositides at the neuromuscular junction of Drosophila melanogaster: a genetic approach
Authors: Slabbaert, Jan ×
Khuong, Thang Manh
Verstreken, Patrik #
Issue Date: 2012
Publisher: Academic Press
Series Title: Methods in Cell Biology vol:108 pages:227-247
Abstract: Phosphoinositides are critically important for numerous cellular signaling pathways such as membrane trafficking, cytoskeleton rearrangement, and ion channel regulation in eukaryotic organisms. The physiological relevance of phosphoinositide metabolism at the Drosophila neuromuscular junction (NMJ) has been illustrated using several mutants that lack crucial factors of phosphoinositide signaling. Although several decades of research in both in vitro and in vivo models have led to an understanding of the mechanisms of lipid-protein interactions and downstream signaling, the details on how their temporal and spatial distribution is regulated at the sub-cellular level in vivo remains poorly understood. To obtain a better understanding of phosphoinositide signaling, detailed biochemical and cell biological approaches can best be combined with genetics. In this review, we present an overview of the methodologies available in the fruit fly Drosophila melanogaster, to genetically dissect the complex regulation of signaling pathways involving phosphoinositides.
URI: 
ISSN: 0091-679X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Neuronal Communication
Department of Human Genetics - miscellaneous
× corresponding author
# (joint) last author

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