Title: Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system
Authors: Stynen, Bram
Tournu, Hélène
Tavernier, Jan
Van Dijck, Patrick # ×
Issue Date: Jun-2012
Publisher: American Society for Microbiology (ASM)
Series Title: Microbiology and Molecular Biology Reviews vol:76 issue:2 pages:331-382
Abstract: The yeast two-hybrid system pioneered the field of in vivo protein-protein interaction methods and undisputedly gave rise to a palette of ingenious techniques that are constantly pushing further the limits of the original method. Sensitivity and selectivity have improved because of various technical tricks and experimental designs. Here we present an exhaustive overview of the genetic approaches available to study in vivo binary protein interactions, based on two-hybrid and protein fragment complementation assays. These methods have been engineered and employed successfully in microorganisms such as Saccharomyces cerevisiae and Escherichia coli, but also in higher eukaryotes. From single binary pairwise interactions to whole-genome interactome mapping, the self-reassembly concept has been employed widely. Innovative studies report the use of proteins such as ubiquitin, dihydrofolate reductase, and adenylate cyclase as reconstituted reporters. Protein fragment complementation assays have extended the possibilities in protein-protein interaction studies, with technologies that enable spatial and temporal analyses of protein complexes. In addition, one-hybrid and three-hybrid systems have broadened the types of interactions that can be studied and the findings that can be obtained. Applications of these technologies are discussed, together with the advantages and limitations of the available assays.
ISSN: 1092-2172
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Microbiology and Biotechnology Section - miscellaneous (-)
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Microbiol. Mol. Biol. Rev.-2012-Stynen-331-82.pdf Published 9499KbAdobe PDFView/Open


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science