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Title: Efficient secretion of biologically active mouse tumor necrosis factor alpha by Streptomyces lividans
Authors: Van Mellaert, Lieve ×
Dillen, C
Proost, Paul
Sablon, E
DeLeys, R
Van Broekhoven, A
Heremans, Hubertine
Van Damme, Jozef
Eyssen, Hendrik
Anné, Jozef #
Issue Date: Dec-1994
Series Title: Gene vol:150 issue:1 pages:153-8
Abstract: We have studied the production of mouse tumor necrosis factor alpha (mTNF) with Streptomyces lividans as host. mTNF cDNA was fused to the alpha-amylase-encoding gene (aml) of Streptomyces venezuelae ATCC15068 at 12 amino acids (aa) downstream from the signal-peptidase cleavage site so that the aa surrounding this processing site were conserved. S. lividans containing this construct secreted mTNF at moderately high levels (1-10 micrograms/ml) as a biologically active compound of high specific activity (1 x 10(8) units/mg protein). No unprocessed pre-protein and virtually no processed protein could be detected in the cell lysates. N-terminal aa sequence analysis indicated microheterogeneity (-3 to -6 forms) at the N-terminal site of secreted mTNF. It was demonstrated that this microheterogeneity was due to aminopeptidase activity.
ISSN: 0378-1119
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Academic Center for General Practice
Laboratory of Molecular Immunology (Rega Institute)
Laboratory of Molecular Bacteriology (Rega Institute)
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

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