Title: Isolation, primary structure and synthesis of neomyosuppressin, a myoinhibiting neuropeptide from the grey fleshfly, Neobellieria bullata
Authors: Fónagy, A ×
Schoofs, Liliane
Proost, Paul
Van Damme, Jozef
Bueds, H
De Loof, Arnold #
Issue Date: Jun-1992
Publisher: Pergamon
Series Title: Comparative Biochemistry and Physiology C, Comparative Pharmacology and Toxicology vol:102 issue:2 pages:239-245
Abstract: 1. An amidated decapeptide, showing strong inhibitory activity of spontaneous visceral muscle movement was isolated, from head extracts of 42 thousand fleshflies, Neobellieria bullata (Diptera, Sarcophagidae). 2. Amino acid sequencing and verification by peptide synthesis revealed the following primary structure: Thr-Asp-Val-Asp-His-Val-Phe-Leu-Arg-PheNH2. 3. The novel peptide was termed neomyosuppressin or Neb-MS. 4. During the process of consecutive high performance liquid chromatography (HPLC) purifications the biological activity of the samples was monitored using heterologous bioassay system. 5. The threshold level of synthetic Neb-MS was found to be 8.6 +/- 0.5 x 10(-11) M on the Leucophaea hindgut and 3.4 +/- 0.5 x 10(-10) M on the Locusta oviduct bioassay, respectively.
ISSN: 0742-8413
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Animal Physiology and Neurobiology Section - miscellaneous
Academic Center for General Practice
Laboratory of Molecular Immunology (Rega Institute)
Department of Biology - miscellaneous
× corresponding author
# (joint) last author

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