Title: Characterization of herpes simplex virus type 1 thymidine kinase mutants selected under a single round of high-dose brivudin
Authors: Andrei, Graciela ×
Balzarini, Jan
Fiten, Pierre
De Clercq, Erik
Opdenakker, Ghislain
Snoeck, Robert #
Issue Date: May-2005
Publisher: American Society for Microbiology (ASM)
Series Title: Journal of Virology vol:79 issue:9 pages:5863-5869
Abstract: A broad variety of herpes simplex virus type 1 clones was selected under a single round of high-dose selection with brivudin. Mutations in the thymidine kinase (TK) genes consisted of 42% frameshift mutations within homopolymer repeats of G's and C's and single nucleotide substitutions (58%) that produced stop codons (Q261 and R281) or a new codon at the site of the substitution (A168T, R51W, G59W, G206R, R220H, Y239S, and T287 M). The A168T change, associated with an altered TK phenotype, proved to be the most commonly selected substitution. For the different mutants, a correlation between phenotype, genotype, and in vivo neurovirulence was observed.
ISSN: 0022-538X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Virology and Chemotherapy (Rega Institute)
Laboratory of Immunobiology (Rega Institute)
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science