Title: Evaluation of TatABC overproduction on Tat- and Sec-dependent protein secretion in Streptomyces lividans
Authors: De Keersmaeker, Sophie ×
Vrancken, Kristof
Van Mellaert, Lieve
Lammertyn, Elke
Anné, Jozef
Geukens, Nick #
Issue Date: Dec-2006
Series Title: Archives of microbiology vol:186 issue:6 pages:507-12
Abstract: The majority of bacterial proteins are exported across the cytoplasmic membrane via the Sec pathway, but also the more recently discovered twin-arginine translocation (Tat) route seems to play an important role for protein secretion in Streptomyces lividans in whose genome tatA, tatB and tatC have been identified. In the present work we showed that simultaneous overproduction of TatABC improved the Tat-dependent secretion capacity as could be concluded from the increased amount of secreted xylanase C, an exclusive Tat-dependent substrate. This result demonstrates that next to the availability of energy to drive secretion, also the number of translocases can be rate-limiting for Tat-dependent secretion. On the other hand, tatABC overexpression was found to diminish secretion of the Sec-dependent proteins xylanase B and subtilisin inhibitor in S. lividans. These results reveal cross-talk between both pathways in S. lividans.
ISSN: 0302-8933
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Molecular Bacteriology (Rega Institute)
Laboratory of Virology and Chemotherapy (Rega Institute)
× corresponding author
# (joint) last author

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