Rapid identification of bacteria by real-time amplification and sequencing of the 16S rRNA gene
Vliegen, Inge × Jacobs, Jan A Beuken, Erik Bruggeman, Cathrien A Vink, Cornelis #
Journal of microbiological methods vol:66 issue:1 pages:156-64
To allow rapid identification of bacteria in pure cultures and blood culture bottles, an assay was developed which is based on real-time amplification and sequencing of bacterial 16 S rRNA genes. In principle, this assay allows identification of bacteria from pure cultures within 6.5 h, and from blood cultures within approximately 7 h.