Title: Growth of Azospirillum irakense KBC1 on the aryl beta-glucoside salicin requires either salA or salB
Authors: Faure, D. ×
Desair, Josephus
Keijers, V.
Bekri, M. A.
Proost, Paul
Henrissat, B.
Vanderleyden, Jozef #
Issue Date: May-1999
Series Title: Journal of bacteriology vol:181 issue:10 pages:3003-3009
Abstract: The rhizosphere nitrogen-fixing bacterium Azospirillum irakense KBC1 is able to grow on pectin and beta-glucosides such as cellobiose, arbutin, and salicin. Two adjacent genes, salA and salB, conferring beta-glucosidase activity to Escherichia coli, have been identified in a cosmid library of A. irakense DNA. The SalA and SalB enzymes preferentially hydrolyzed aryl beta-glucosides. A Delta(salA-salB) A. irakense mutant was not able to grow on salicin but could still utilize arbutin, cellobiose, and glucose for growth. This mutant could be complemented by either salA or salB, suggesting functional redundancy of these genes in salicin utilization. In contrast to this functional homology, the SalA and SalB proteins, members of family 3 of the glycosyl hydrolases, show a low degree of amino acid similarity. Unlike SalA, the SalB protein exhibits an atypical truncated C-terminal region. We propose that SalA and SalB are representatives of the AB and AB' subfamilies, respectively, in glycosyl hydrolase family 3. This is the first genetic implication of this beta-glucosidase family in the utilization of beta-glucosides for microbial growth.
ISSN: 0021-9193
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
Laboratory of Molecular Immunology (Rega Institute)
× corresponding author
# (joint) last author

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