Title: Identification by two-dimensional electrophoresis of a new adhesin expressed by a low-passaged strain of Mycoplasma bovis
Authors: Thomas, A
Leprince, P
Dizier, I
Ball, H
Gevaert, K
Van Damme, Jozef
Mainil, J
Linden, A #
Issue Date: Jun-2005
Publisher: Elsevier science bv
Series Title: Research in microbiology vol:156 issue:5-6 pages:713-718
Abstract: A significant decrease in adherence rates of Mycoplasma bovis to bovine bronchial epithelial (BBE) cells has been observed after passage of the organism in artificial medium. Analysis of the proteins expressed by M. bovis isolate 2610 by two-dimensional (2-D) electrophoresis demonstrated differences between the cells harvested after the 7th and 116th passage. Three silver-stained prominent spots observed in 2-D electrophoretic separation of protein extracts of the lower-passaged cells were considerably less strongly expressed in the sample from higher-passaged cells. These spots had a molecular mass of approximately 24 kDa and an isoelectric point of about 5. The mass spectrometry analysis of these trypsin-sensitive proteins led to their identification as a unique new member of the Vsps family of membrane-associated proteins. Serum from a mouse immunized with these proteins significantly reduced adherence of M. bovis to BBE cells. This result underlines the function of this new Vsp in adherence of M. bovis to host cells. (c) 2005 Elsevier SAS. All rights reserved.
ISSN: 0923-2508
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Arts, Campus Kulak Kortrijk
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science