Title: Thymidine analogs are transferred from prelabeled donor to host cells in the central nervous system after transplantation: a word of caution
Authors: Burns, Terry C ×
Ortiz-González, Xilma R
Gutiérrez-Pérez, María
Keene, C Dirk
Sharda, Rohit
Demorest, Zachary L
Jiang, Yuehua
Nelson-Holte, Molly
Soriano, Mario
Nakagawa, Yasushi
Luquin, María Rosario
Garcia-Verdugo, Jose Manuel
Prósper, Felipe
Low, Walter C
Verfaillie, Catherine #
Issue Date: Apr-2006
Publisher: AlphaMed Press
Series Title: Stem Cells vol:24 issue:4 pages:1121-1127
Abstract: Thymidine analogs, including bromodeoxyuridine, chlorodeoxyuridine, iododeoxyuridine, and tritiated thymidine, label dividing cells by incorporating into DNA during S phase of cell division and are widely employed to identify cells transplanted into the central nervous system. However, the potential for transfer of thymidine analogs from grafted cells to dividing host cells has not been thoroughly tested. We here demonstrate that graft-derived thymidine analogs can become incorporated into host neural precursors and glia. Large numbers of labeled neurons and glia were found 3-12 weeks after transplantation of thymidine analog-labeled live stem cells, suggesting differentiation of grafted cells. Remarkably, however, similar results were obtained after transplantation of dead cells or labeled fibroblasts. Our findings reveal for the first time that thymidine analog labeling may not be a reliable means of identifying transplanted cells, particularly in highly proliferative environments such as the developing, neurogenic, or injured brain.
ISSN: 1066-5099
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

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