Title: Silencing SERCA1b in a few fibers stimulates growth in the entire regenerating soleus muscle
Authors: Zador, Erno ×
Owsianik, Grzegorz
Wuytack, Frank #
Issue Date: Jan-2011
Publisher: Springer-Verlag
Series Title: Histochemistry and Cell Biology vol:135 issue:1 pages:11-20
Abstract: The neonatal isoform of the sarcoplasmic/endoplasmic reticulum Ca2+ ATPase 1 (SERCA1b) is a dominant Ca2+ pump in the young fibers of regenerating muscle. In vivo transfection of about 1% of the fibers with SERCA1b RNAi plasmid resulted in no apparent change in the transfected fibers, but enhanced the increase of fresh weight and fiber size in the whole regenerating rat soleus muscle, until the normal size was reached. Co-transfection of calcineurin inhibitor cain/cabin-1 with SERCA1b RNAi was sufficient to cut down the widespread growth stimulation, but the subsequent transfection of cain into the SERCA1b RNAi transfected muscle did not inhibit muscle growth. The SERCA1b RNAi preferably upregulated the expression of the NFAT reporter lacZ compared to controls when co-transfected into the fibers. Notably, perimuscular injection of interleukin-4 (IL-4) antibody but not that of an unrelevant antibody completely abolished the growth-promoting effect of SERCA1b RNAi. This indicates that silencing SERCA1b in a few fibers stimulates the calcineurin-NFAT-IL-4 pathway and fiber growth in the whole regenerating soleus. These results suggest the presence of an autocrine paracrine coordination of growing muscle fibers, and put forward a new method to stimulate skeletal muscle regeneration.
ISSN: 0948-6143
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Ion Channel Research
Laboratory of Cellular Transport Systems
× corresponding author
# (joint) last author

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