A total proteome map of the Pseudomonas aeruginosa PAO1 proteome is presented,
generated by a combination of two-dimensional gel electrophoresis and
protein identification by mass spectrometry. In total, 1128 spotswere visualized, and
181 protein spotswere characterized, corresponding to 159 different protein entries.
In particular, protein chaperones and enzymes important in energy conversion and
amino acid biosynthesis were identified. Spot analysis always resulted in the identification
of a single protein, suggesting sufficient spot resolution, although the same
proteinmay be detected in two ormore neighboring spots, possibly indicating posttranslational
modifications. Comparison to the theoretical proteome revealed an
underrepresentation of membrane proteins, though the identified proteins cover
all predicted subcellular localizations and all functional classes. These data provide
a basis for subsequent comparative studies of the biology and metabolism of
P. aeruginosa, aimed at unraveling global regulatory networks.