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Title: Simultaneous determination of AMN107 and Imatinib (Gleevec((R)), Glivec((R)), STI571) in cultured tumour cells using an isocratic high-performance liquid chromatography procedure with UV detection
Authors: Guetens, Gunther ×
Prenen, Hans
De Boeck, Geert
Van Oosterom, Allan
Schöffski, Patrick
Highley, Martin
de Bruijn, Ernst #
Issue Date: Feb-2007
Series Title: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences vol:846 issue:1-2 pages:341-5
Abstract: A reversed phase high-performance liquid chromatographic (HPLC) method with UV detection was developed for the simultaneous determination of imatinib (Gleevec((R)), Glivec((R)), STI571) and AMN107 in cultured tumour cells, using clozapine as an internal standard. The compounds of interest were extracted by liquid-liquid extraction using TOXI-TUBES((R)) A extraction tubes. Chromatographic separation was performed on a Phenomenex Gemini C18 reversed phase column (150mmx2.0mm, 5mum particle size), using a mixture of 65% CH(3)OH (methanol) and 35% NH(4)Ac (Ammonium acetate) buffer (20mM, pH 10). Separation was achieved under isocratic conditions at a flow rate of 0.5ml/min. Imatinib, clozapine and AMN107 are detected by UV detection at 260nm. Calibration curves were linear from 50 to 7500ng/ml with correlation coefficients (r(2)) better than 0.998. The limit of quantitation (LOD) was 50ng/ml. The method has been successfully applied to a cellular kinetics study.
ISSN: 1570-0232
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Laboratory of Experimental Oncology
Department of Oncology - miscellaneous
Clinical Digestive Oncology (+)
× corresponding author
# (joint) last author

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