Title: Functionalization of thioctic acid-capped gold nanoparticles for specific immobilization of histidine-tagged proteins
Authors: Abad, JM ×
Mertens, SFL
Pita, M
Fernandez, VM
Schiffrin, DJ #
Issue Date: Apr-2005
Publisher: American Chemical Society
Series Title: Journal of the American Chemical Society vol:127 issue:15 pages:5689-5694
Abstract: This paper presents an efficient strategy for the specific immobilization of fully functional proteins onto the surface of nanoparticles. Thioctic acid-derivatized gold clusters are used as a scaffold for further stepwise modification, leading to a cobalt(II)-terminated ligand shell. A histidine tag introduced by genetic engineering into a protein is coordinated to this transition metal ion. The specific immobilization has been demonstrated for the cases of a genetically engineered horseradish peroxidase and ferredoxin-NADP+ reductase, confirming the attachment of the fully functional proteins to the Co(II)-terminated nanointerface. The absence of nonspecific protein adsorption and the specificity of the binding site have been verified using several analogues of the enzymes without the histidine tag.
ISSN: 0002-7863
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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