Title: Genome-wide reverse genetics framework to identify novel functions of the vertebrate secretome
Authors: Pickart, Michael A ×
Klee, Eric W
Nielsen, Aubrey L
Sivasubbu, Sridhar
Mendenhall, Eric M
Bill, Brent R
Chen, Eleanor
Eckfeldt, Craig E
Knowlton, Michelle
Robu, Mara E
Larson, Jon D
Deng, Yun
Schimmenti, Lisa A
Ellis, Lynda B M
Verfaillie, Catherine
Hammerschmidt, Matthias
Farber, Steven A
Ekker, Stephen C #
Issue Date: Dec-2006
Publisher: Public Library of Sciene
Series Title: PLoS One vol:1 issue:1
Article number: e104
Abstract: BACKGROUND: Understanding the functional role(s) of the more than 20,000 proteins of the vertebrate genome is a major next step in the post-genome era. The approximately 4,000 co-translationally translocated (CTT) proteins - representing the vertebrate secretome - are important for such vertebrate-critical processes as organogenesis. However, the role(s) for most of these genes is currently unknown. RESULTS: We identified 585 putative full-length zebrafish CTT proteins using cross-species genomic and EST-based comparative sequence analyses. We further investigated 150 of these genes (Figure 1) for unique function using morpholino-based analysis in zebrafish embryos. 12% of the CTT protein-deficient embryos resulted in specific developmental defects, a notably higher rate of gene function annotation than the 2%-3% estimate from random gene mutagenesis studies. CONCLUSION#ENTITYSTARTX00028;S#ENTITYSTARTX00029;: This initial collection includes novel genes required for the development of vascular, hematopoietic, pigmentation, and craniofacial tissues, as well as lipid metabolism, and organogenesis. This study provides a framework utilizing zebrafish for the systematic assignment of biological function in a vertebrate genome.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Interdepartemental Stem Cell Institute (-)
× corresponding author
# (joint) last author

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