The limitations imposed to both autogenous and allogenous bone grafts led to the development of new strategies for the treatment of large bone
defects. The approach of bone tissue engineering aims to restore damaged bone tissue by combining osteocompetent cells such as mesenchymal
stromal cells (MSC), and material scaffolds like ceramics. However, the therapeutic effectiveness of cell constructs has not yet met that of autologous
bone grafts, in part due to the high death rate of cells (loaded onto the material scaffold) upon their implantation into the injured site. In order to
improve the therapeutic functionality of these cell constructs, different strategies can be implemented. In this context, the Glassbone project aimed
to optimize the conditions for preparation of tissue engineered products by approaching three aspects: identification of optimal ceramic scaffold
relevant to bone formation; survival of implanted cells post-implantation, and finally cell preconditioning to promote cell viability in vivo. Such
project will pave the way for the development of new “pro-survival” tissue engineered materials for optimal tissue regeneration.