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Title: Genomic cloning, heterologous expression and pharmacological characterization of a human histamine H1 receptor
Authors: De Backer, M D ×
Gommeren, W
Moereels, H
Nobels, G
Van Gompel, P
Leysen, J E
Luyten, Walter #
Issue Date: Dec-1993
Publisher: Academic Press
Series Title: Biochemical and Biophysical Research Communications vol:197 issue:3 pages:1601-8
Abstract: A human histamine H1 receptor gene lacking introns was isolated by screening a human genomic library with a bovine histamine H1 receptor probe. The deduced protein of 487 amino acids showed characteristic properties of G-protein-coupled receptors. The coding region was subcloned into the expression vector pSVL (Pharmacia), and the resulting construct transfected into COS-7 cells. Binding studies with [3H]pyrilamine on membranes from transfected cells revealed saturable specific binding with a KD of 1.2 nM and a Bmax of 3400 fmol/mg protein. Binding affinities of histamine and known histamine antagonists were similar to those for histamine H1 receptors in guinea-pig cerebellum. In transfected COS-7 cells, histamine induced inositol phosphate formation, that was inhibitable by pyrilamine.
ISSN: 0006-291X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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