A human homologue of the checkpoint kinase Cds1 directly inhibits Cdc25 phosphatase
Blasina, A × de Weyer, I V Laus, M C Luyten, Walter Parker, A E McGowan, C H #
Current Biology Ltd.
Current Biology vol:9 issue:1 pages:1-10
In human cells, the mitosis-inducing kinase Cdc2 is inhibited by phosphorylation on Thr14 and Tyr15. Disruption of these phosphorylation sites abrogates checkpoint-mediated regulation of Cdc2 and renders cells highly sensitive to agents that damage DNA. Phosphorylation of these sites is controlled by the opposing activities of the Wee1/Myt1 kinases and the Cdc25 phosphatase. The regulation of these enzymes is therefore likely to be crucial for the operation of the G2-M DNA-damage checkpoint.