Title: The hepatocarcinoma cell line HepG2 does not express a GHS-R1a-type ghrelin receptor
Authors: Thielemans, Leen ×
Peeters, Pieter J
Jonckheere, Heidi
Luyten, Walter
de Hoogt, Ronald
Coulie, Bernard
Aerssens, Jeroen #
Issue Date: 2007
Publisher: Marcel Dekker
Series Title: Journal of Receptor and Signal Transduction Research vol:27 issue:4 pages:309-322
Abstract: The interaction of ghrelin, a 28-residue acylated peptide, with the growth hormone secretagogue receptor 1a (GHS-R1a) has been studied mostly in cells expressing a recombinant GHS-R1a. As awareness is growing on the importance to study G protein-coupled receptors in a natural environment, we studied the effect of ghrelin in the human hepatocellular HepG2 cell line because it has been described in literature to respond to ghrelin. Despite extensive efforts, we were not able to confirm mRNA expression of GHS-R1a by reverse transcription PCR, radioligand binding, or ghrelin-induced GHS-R1a receptor activation; therefore, we conclude that HepG2 cells do not express GHS-R1a. On the other hand, we confirmed a modest effect of ghrelin on the up-regulation of IRS-1 phosphorylation, which might suggest the existence of an alternative ghrelin receptor in HepG2 cells.
ISSN: 1079-9893
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Translational Research in GastroIntestinal Disorders
Department of Pharmaceutical & Pharmacological Sciences - miscellaneous
× corresponding author
# (joint) last author

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