Title: Limited proteolysis of the hemocyanin of the gastropod pila-leopoldvillensis - isolation and characterization of the fragments
Authors: Gielens, Constant ×
Declercq, L
Preaux, G #
Issue Date: Jan-1995
Publisher: Pergamon-elsevier science ltd
Series Title: Comparative biochemistry and physiology b-biochemistry & molecular biology vol:110 issue:3 pages:565-575
Abstract: Limited proteolysis with trypsin and endoproteinase Glu-C of dimers of subunits of the haemocyanin of Pila leopoldvillensis revealed the presence of eight functional (dioxygen binding) units (a-h) per subunit (M(r) approximate to 435,000). Fragments a-c, d-g and (d-h)(2) (dimer of d-h) and the dimer of functional unit h (h(2)) were isolated and characterized (copper content, M(r), N- and C-terminus, crossed immunoelectrophoresis, absorption and circular dichroic spectra, carbohydrate composition). Functional unit h contains the association site between the two subunits in a dimer, When compared with the average values for a functional unit it, moreover, shows a higher M(r) (approximate to 65,000 vs approximate to 55,000) and a higher carbohydrate content (approximate to 5.5%, w/w, vs approximate to 3.5%).
ISSN: 0305-0491
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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