Title: Recent developments in quantification methods for metallothionein
Authors: Dabrio, M ×
Rodriguez, AR
Bordin, G
Bebianno, MJ
De Ley, Marc
Sestakova, I
Vasak, M
Nordberg, M #
Issue Date: Jan-2002
Publisher: Elsevier science inc
Series Title: Journal of inorganic biochemistry vol:88 issue:2 pages:123-134
Abstract: The metallothioneins (MT), a family of proteins with relatively low molecular weight (6-7 kDa), are characterised by the intrinsic presence of 20 cysteinyl groups in their structure, which confers unique metal binding properties to the molecule. Since MT are involved in biological roles, quantification of MT remains an important task. To date, a large number of determination methods have been developed. In this paper recent developments, from 1995 to the present, in methodology employed in quantification studies of total NIT and MT polymorphism are described. Different fields were taken into consideration, such as (i) separation techniques and hyphenated systems, (ii) electrochemical methods, (iii) immunological methods and (iv) quantification of MT mRNA. The data presented are based on our own and published results. A brief overview of the use of metallothionein as a biomarker is included as a relevant example of the importance of MT quantification. Finally, general problems associated with determination and evaluation of obtained results within the above four topics are mentioned. (C) 2002 Elsevier Science B.V. All rights reserved.
ISSN: 0162-0134
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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