Single-Step Antigen Loading and Activation of Dendritic Cells by mRNA Electroporation for the Purpose of Therapeutic Vaccination in Melanoma Patients
Bonehill, Aude × Van Nuffel, An M. T Corthals, Jurgen Tuyaerts, Sandra Heirman, Carlo Francois, Violaine Colau, Didier van der Bruggen, Pierre Neyns, Bart Thielemans, Kris #
Association for Cancer Research
Clinical Cancer Research vol:15 issue:10 pages:3366-3375
Purpose: A critical factor determining the effectiveness of currently used dendritic cell (DC)-based vaccines is the DC activation or maturation status. We have recently shown that the T-cell stimulatory capacity of DCs pulsed with tumor-antigen-derived peptides can be considerably increased by activating the DCs through electroporation with mRNA encoding CD40 ligand, CD70, and a constitutively active Toll-like receptor 4 (TriMix DCs). Here, we investigate whether TriMix DCs can be coelectroporated with whole tumor-antigen-encoding mRNA.