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Title: Conversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by MLL Partner TET1
Authors: Tahiliani, Mamta
Koh, Kian
Shen, Yinghua
Pastor, William A
Bandukwala, Hozefa
Brudno, Yevgeny
Agarwal, Suneet
Iyer, Lakshminarayan M
Liu, David R
Aravind, L
Rao, Anjana # ×
Issue Date: 15-May-2009
Publisher: American Association for the Advancement of Science
Series Title: Science vol:324 issue:5929 pages:930-935
Abstract: DNA cytosine methylation is crucial for retrotransposon silencing and mammalian development. In a computational search for enzymes that could modify 5-methylcytosine (5mC), we identified TET proteins as mammalian homologs of the trypanosome proteins JBP1 and JBP2, which have been proposed to oxidize the 5-methyl group of thymine. We show here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. hmC is present in the genome of mouse embryonic stem cells, and hmC levels decrease upon RNA interference-mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC.
URI: 
ISSN: 0036-8075
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Embryo and Stemcells (-)
× corresponding author
# (joint) last author

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