ITEM METADATA RECORD
Title: Domain Requirements and Sequence Specificity of DNA Binding for the Forkhead Transcription Factor FOXP3
Authors: Koh, Kian *
Sundrud, Mark S *
Rao, Anjana # ×
Issue Date: 1-Dec-2009
Publisher: Public Library of Sciene
Series Title: PLoS One vol:4 issue:12 pages:-
Article number: e8109
Abstract: The forkhead, winged-helix transcription factor FOXP3 is preferentially expressed in T regulatory (Treg) cells and is critical for their immunosuppressive function. Mutations that abolish FOXP3 function lead to systemic autoimmunity in mice and humans. However, the manner by which FOXP3 recognizes cognate DNA elements is unclear. Here we identify an in vitro optimized DNA sequence to assess FOXP3 DNA binding by electrophoretic mobility shift assay (EMSA). The optimized sequence contains two tandem copies of a core DNA element resembling, but not identical to, the canonical forkhead (FKH) binding element. The tandem nature of this optimized FOXP3-binding oligonucleotide suggests a requirement for multimerization, and EMSA experiments confirm that both the DNA-binding FKH domain and an intact leucine-zipper domain, which mediates homo-multimerization of FOXP3, are required for DNA binding. These results establish a practical framework for understanding the molecular basis by which FOXP3 regulates gene transcription and programs Treg suppressive function.
ISSN: 1932-6203
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Embryo and Stemcells (-)
* (joint) first author
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy

 




All items in Lirias are protected by copyright, with all rights reserved.

© Web of science