Journal of Medical Microbiology vol:61 issue:6 pages:813-819
We investigated the molecular basis of the tolerance of Candida albicans biofilms to the model compound miconazole, and translated these data to other antifungals. Sessile cells of C. albicans Δefg1, lacking the transcription factor Efg1, showed increased susceptibility to miconazole, amphotericin B and caspofungin, whereas these sessile cells were equally resistant to fluconazole. The increased miconazole sensitivity was at least partly due to an increased accumulation of miconazole in the cells as compared to wild type or reintegrant Δefg1(EFG1) sessile cells. By using a rat biofilm model, we further confirmed the role of Efg1 in miconazole tolerance of in vivo grown C. albicans biofilms.