Title: Hypoxic regulation of NF-kappa B signaling
Authors: Cummins, Eoin P
Comerford, Katrina M
Scholz, Carsten
Taylor, Cormac T # ×
Issue Date: 2007
Publisher: Academic Press
Series Title: Methods in Enzymology vol:435 pages:479-492
Abstract: Hypoxia and inflammation are coincidental events in an array of diseased tissues, including chronically inflamed sites (e.g., inflammatory bowel disease, rheumatoid arthritis), growing tumors, myocardial infarcts, atherosclerotic plaques, heating wounds, and sites of bacterial infection (Murdoch et al., 2005). An understanding of how hypoxia modulates the inflammatory response is critical in developing our fundamental understanding of inflammatory disease and identifying new windows of therapeutic opportunity. Nuclear factor-kappa B (NF-kappa B) is a master transcriptional regulator of inflammatory and antiapoptotic gene expression, the activation of which has significant implications in disease development. Recent work has uncovered mechanisms by which hypoxia modulates the activation of NF-kappa B in cells through decreased oxygen-dependent suppression of the key regulators of this pathway. This work has implicated a novel role for proline and asparagine hydroxylases in the modulation of NF-kappa B activity. Here, we describe methodologies used to demonstrate and interrogate hypoxic induction of the NF-kappa B pathway.
ISSN: 0076-6879
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Non-KU Leuven Association publications
× corresponding author
# (joint) last author

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