Title: Simultaneous liquid chromatography determination of polyamines and arylalkyl monoamines
Authors: Geuns, Jan ×
Orriach, ML
Swennen, Rony
Zhu, GY
Panis, Bart
Compernolle, Frans
Van der Auweraer, Mark #
Issue Date: Jul-2006
Publisher: Academic press inc elsevier science
Series Title: Analytical Biochemistry vol:354 issue:1 pages:127-131
Abstract: The diamines putrescine (PUT) and diaminopropane (DAP), the polyamines spermidine (SPD) and spermine (SPM), and the arylalkyl amines phenethylamine (PEA), tyramine (TYR), dopamine (DA), and salsolinol (SAL) were dansylated and baseline separated by LC using a Waters ODS-2 column. The dansyl derivatives were detected by fluorescence (lambda(ex): 337 nm; lambda(em): 520 nm). Besides the amine function, the phenolic OH groups of TYR, DA, and SAL were also dansylated (LC-MS, formation of NO-didansyl [TYR] and A O,O'-tridansyl derivatives [DA and SAL]). Calibration curves revealed response factors being appreciably lower for (N,O-didansyl) aminophenol TYR and (N, O, O'-tridansyl) DA and SAL than for N-dansylamines. However, the method is suitable as a cheap alternative to LC-MS for the simultaneous determination of polyamines and arylalkyl amines of large quantities of samples. (c) 2006 Elsevier Inc. All rights reserved.
ISSN: 0003-2697
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Division of Crop Biotechnics
Molecular Design and Synthesis
Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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