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Title: Spectroscopic characterization of Venus at the single molecule level
Authors: David, Charlotte
Dedecker, Peter
De Cremer, Gert
Verstraeten, Natalie
Kint, Cyrielle
Michiels, Jan
Hofkens, Johan # ×
Issue Date: Feb-2012
Publisher: Royal Society of Chemistry
Series Title: Photochemical & Photobiological Sciences vol:11 issue:2 pages:358-363
Abstract: Venus is a recently developed, fast maturating, yellow fluorescent protein that has been used as a probe for in vivo applications. In the present work the photophysical characteristics of Venus were analyzed spectroscopically at the bulk and single molecule level. Through time-resolved single molecule measurements we found that single molecules of Venus display pronounced fluctuations in fluorescence emission, with clear fluorescence on- and off-times. These fluorescence intermittencies were found to occupy a broad range of time scales, ranging from milliseconds to several seconds. Such long off-times can complicate the analysis of single molecule counting experiments or single-molecule FRET experiments.
URI: 
ISSN: 1474-905X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Molecular Imaging and Photonics
× corresponding author
# (joint) last author

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