Title: Pressure activation of the chaperone function of small heat shock proteins
Authors: Tolgyesi, F ×
Bode, CS
Smeller, L
Kim, DR
Kim, KK
Heremans, Karel
Fidy, J #
Issue Date: Jan-2004
Publisher: Cellular & molecular biology
Series Title: Cellular and molecular biology vol:50 issue:4 pages:361-369
Abstract: Small heat shock proteins play an important role in the stress response of cells and in several other cellular functions. They possess chaperone-like activity; i.e. they can bind and protect damaged proteins from aggregation and maintain them in a folding competent state. Two members of this family were investigated in Us work: bovine (x-crystallin and heat shock protein (HSP) 16.5 from the thermophilic archaebacteria Methanococcus jannaschii. We reported earlier the enhancement of chaperone potency of alpha-crystallin by high pressure. We now report the completion of the work with results on HSP16.5. The chaperone potency of both proteins can be enhanced significantly by applying high pressure. Evidence by light scattering, Fourier transform infrared (FT-IR) and tryptophan fluorescence experiments show that while the secondary and tertiary structure of these proteins are not influenced by high pressure, their quaternary structure becomes affected: H bonds between subunits are weakened or broken, tryptophan environments become more polar, oligomers dissociate to some extent. We conclude that the oligomeric structure of both proteins is loosened, resulting in stronger dynamics and in more accessible hydrophobic surfaces. These properties lead to increased chaperone potency.
ISSN: 0145-5680
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Biochemistry, Molecular and Structural Biology Section
× corresponding author
# (joint) last author

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