ITEM METADATA RECORD
Title: Single-cell recording and stimulation with a 16k micro-nail electrode array integrated on a 0.18 μm CMOS chip
Authors: Huys, Roeland ×
Braeken, Dries
Jans, Danny
Stassen, Andim
Collaert, Nadine
Wouters, Jan
Loo, Josine
Severi, Simone
Vleugels, Frank
Callewaert, Geert
Verstreken, Kris
Bartic, Carmen
Eberle, Wolfgang #
Issue Date: Apr-2012
Publisher: Royal Society of Chemistry
Series Title: Lab on a chip vol:12 issue:7 pages:1274-1280
Abstract: To cope with the growing needs in research towards the understanding of cellular function and network dynamics, advanced micro-electrode arrays (MEAs) based on integrated complementary metal oxide semiconductor (CMOS) circuits have been increasingly reported. Although such arrays contain a large number of sensors for recording and/or stimulation, the size of the electrodes on these chips are often larger than a typical mammalian cell. Therefore, true single-cell recording and stimulation remains challenging. Single-cell resolution can be obtained by decreasing the size of the electrodes, which inherently increases the characteristic impedance and noise. Here, we present an array of 16 384 active sensors monolithically integrated on chip, realized in 0.18 μm CMOS technology for recording and stimulation of individual cells. Successful recording of electrical activity of cardiac cells with the chip, validated with intracellular whole-cell patch clamp recordings are presented, illustrating single-cell readout capability. Further, by applying a single-electrode stimulation protocol, we could pace individual cardiac cells, demonstrating single-cell addressability. This novel electrode array could help pave the way towards solving complex interactions of mammalian cellular networks.
URI: 
ISSN: 1473-0197
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Faculty of Medicine @ Kulak
Physiology, Kulak (-)
Solid State Physics and Magnetism Section
× corresponding author
# (joint) last author

Files in This Item:
File Description Status SizeFormat
Lab_on_chip_2012.pdf Accepted 636KbAdobe PDFView/Open

These files are only available to some KU Leuven staff members

 


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science