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Keywords:

osteoclastogenesis, icam-1, co-culture, f-actin, co-localization, function-associated antigen-1, lymphocyte migration, osteoblastic cells, bone-cells, in-vitro, involvement, precursors, pathway, Science & Technology, Life Sciences & Biomedicine, Biochemistry & Molecular Biology, Biophysics, Osteoclastogenesis, ICAM-1, Co-culture, F-actin, Co-localization, FUNCTION-ASSOCIATED ANTIGEN-1, LYMPHOCYTE MIGRATION, OSTEOBLASTIC CELLS, BONE-CELLS, IN-VITRO, INVOLVEMENT, PRECURSORS, PATHWAY, Actins, Cell Adhesion, Cell Differentiation, Cell Movement, Cells, Cultured, Humans, Intercellular Adhesion Molecule-1, Osteoclasts, Stem Cells, 0304 Medicinal and Biomolecular Chemistry, 0601 Biochemistry and Cell Biology, 1101 Medical Biochemistry and Metabolomics, 3101 Biochemistry and cell biology, 3404 Medicinal and biomolecular chemistry

Abstract:

Adhesion between osteoblasts and osteoclast precursors is established via an interaction involving intercellular adhesion molecule-1 (ICAM-1) on osteoblasts and leukocyte function-associated antigen-1 (LFA-1) on osteoclast precursors. The latter cells also express ICAM-1, but little is known about the expression over time and its possible role during osteoclastogenesis. In the present Study we investigated the expression of ICAM-1 on both human osteoblast-like cells and osteoclast precursors in a co-culture. The protein expression on osteoclast precursors strongly increased whereas the osteoblast-like cells became ICAM-1 negative. Interestingly, ICAM-1 on osteoclast precursors manifested as Clusters which localized at the baso-lateral membrane. Furthermore, clustered ICAM-1 was associated with F-actin and remained present for several days. Our data Suggest that osteoblastic ICAM-1 is mainly involved in the initial adhesion of osteoclast precursors whereas Clustered ICAM-1 on osteoclast precursors and its association with F-actin suggest an involvement in cell movement at a later stage. (C) 2009 Elsevier Inc. All rights reserved.