Title: The plant defensin RsAFP2 induces cell wall stress, septin mislocalization and accumulation of ceramides in Candida albicans
Authors: Thevissen, Karin ×
de Mello Tavares, P.
Xu, D.
Blankenship, J.
Vandenbosch, D.
Idkowiak-Baldys, J.
Govaert, Gilmer
Bink, Anna
Rozental, S.
de Groot, P.W.J.
Davis, T.R.
Kumamoto, C.A.
Vargas, G.
Nimrichter, L.
Coenye, T.
Mitchell, A.
Roemer, T.
Hannun, Y.A.
Cammue, Bruno #
Issue Date: Apr-2012
Publisher: Blackwell Science
Series Title: Molecular Microbiology vol:84 issue:1 pages:166-180
Abstract: The antifungal plant defensin RsAFP2 isolated from radish interacts with fungal glucosylceramides and
induces apoptosis in Candida albicans. To further unravel the mechanism of RsAFP2 antifungal action
and tolerance mechanisms, we screened a library of 2,868 heterozygous C. albicans deletion mutants and
identified 30 RsAFP2-hypersensitive mutants. The most prominent group of RsAFP2 tolerance genes was
involved in cell wall integrity and hyphal growth/septin ring formation. Consistent with these genetic
data, we demonstrated that RsAFP2 interacts with the cell wall of C. albicans, which also contains
glucosylceramides, and activates the cell wall integrity pathway. Moreover, we found that RsAFP2
induces mislocalization of septins and blocks the yeast-to-hypha transition in C. albicans. Increased
ceramide levels have previously been shown to result in apoptosis and septin mislocalization. Therefore,
ceramide levels in C. albicans membranes were analyzed following RsAFP2 treatment and, as expected,
increased accumulation of phytoC24-ceramides in membranes of RsAFP2-treated C. albicans cells was
detected. This is the first report on the interaction of a plant defensin with glucosylceramides in the fungal
cell wall, causing cell wall stress, and on the effects of a defensin on septin localization and ceramide
ISSN: 0950-382X
Publication status: published
KU Leuven publication type: IT
Appears in Collections:Centre of Microbial and Plant Genetics
× corresponding author
# (joint) last author

Files in This Item:

There are no files associated with this item.

Request a copy


All items in Lirias are protected by copyright, with all rights reserved.

© Web of science